Vaccine Design for Marburg Virus Using VP35 Protein
Mohammed Yousif Mohammed,
Mona Abdelrhman Mohamed Khaier,
Nuha Agabna,
Sania Shaddad
Issue:
Volume 10, Issue 4, December 2022
Pages:
85-93
Received:
22 October 2022
Accepted:
8 December 2022
Published:
28 December 2022
DOI:
10.11648/j.ijgg.20221004.11
Downloads:
Views:
Abstract: Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.
Abstract: Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that c...
Show More
The Effect of Non-synonymous Single Nucleotide Polymorphisms Variants in ALPL Gene, a Computational Approach
Nabaa Kamal Alshafei,
Intisar Hassan Saeed,
Mona Abdelrahman Mohamed Khaier
Issue:
Volume 10, Issue 4, December 2022
Pages:
94-102
Received:
6 July 2022
Accepted:
26 September 2022
Published:
29 December 2022
DOI:
10.11648/j.ijgg.20221004.12
Downloads:
Views:
Abstract: Background: Phosphorus is one of the major macronutrient essential for normal growth and development of living organisms. Alkaline phosphatase (ALP) is an enzyme present in human and animals responsible for solubilization and mineralization of organic phosphate and makes it readily available for the body. The disease Hypophosphatasia (HPP) is an autosomal recessive inherited one, branded by malfunctioning mineralization of bone, dental problems, and low serum ALP levels. This study aimed to scrutinize the effect of non-synonymous SNPs (nsSNPs) of ALPL gene on protein function and structure using different computational software. Material and Methods: Different Non-Synonymous Single Nucleotide Polymorphisms (nsSNPs) and protein related sequences were attained from NCBI and ExPASY databases. Deleterious and damaging effect of nsSNPs were analyzed using SIFT, Polyphen-2, Provean and SNPs&GO software. Protein stability was inspected using I-Mutant and MUpro software. The interaction of ALPL with other genes was studied using GeneMANIA software. The structural and functional influence of point mutations was predicted using Project Hope software. Results: ALPL gene was found to have an association with 20 other genes such as TRAF3 and TPP1 using GeneMANIA. It comprises a total of 485 SNPs out of that 188 were found to be synonymous, 298 were nsSNPs. Analysis of the nsSNPs by SIFT predicts 33 as deleterious and 265 as tolerated ones. Using Provean software, 26 were deleterious while 7 nsSNPs were neutral. Taking the deleterious nsSNPSs to Polyphen-2, 24 nsSNPs were damaging, while 2 were benign. Using SNPs&GO 13 nsSNPs were predicted as disease-related while 11 were predicted to be neutral. By using PHD SNPs 11 nsSNPs were predicted as disease-related while 13 were predicted to be neutral. Project Hope analyzes the mutations according to their size, charge, hydrophobicity, and conservancy. Conclusion: This study reveals 11 nsSNPs as being possibly pathogenic variants. Seven of them were already reported from previous studies by DNA sequencing, while the remaining four were predicted in this study for the first time.
Abstract: Background: Phosphorus is one of the major macronutrient essential for normal growth and development of living organisms. Alkaline phosphatase (ALP) is an enzyme present in human and animals responsible for solubilization and mineralization of organic phosphate and makes it readily available for the body. The disease Hypophosphatasia (HPP) is an au...
Show More
