Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication
Daniel Azubuike Awomukwu,
Bio Louis Nyananyo,
Paula Spies,
Bulelani Londoloza Sizani
Issue:
Volume 3, Issue 1, February 2015
Pages:
1-7
Received:
30 January 2015
Accepted:
11 February 2015
Published:
16 February 2015
Abstract: An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on GenBank and BoldSystems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and BoldSystems while P. amarus Schum and Thonn, P. muellerianus (O. Ktze) Excel and P. urinaria Linn. were clearly compatible with other works. The sequence data were analyzed and classified with tree-based analyses of Mr.Bayes 3.2.1. in order to reveal their phylogenetic relationship. Results of the nucleotide sequences and fragment analysis were published on BoldSystems for barcoding as non-coding marker translation matrix.
Abstract: An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Trans...
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Distribution of ABO and Rh Blood Groups Among Students of Some Ethnic Groups at Dilla University, Ethiopia
Issue:
Volume 3, Issue 1, February 2015
Pages:
8-19
Received:
4 February 2015
Accepted:
13 February 2015
Published:
26 February 2015
Abstract: Red Blood cells (Erythrocytes) have an inherited combination of proteins, glycol-proteins, and glycol-lipids on their surfaces. These function as antigens that enable our immune system to distinguish our own cells from foreign invaders. Part of the immune response is the production of globulins called antibodies to combat the invader. In blood typing, the antigens of Red Blood Cell surfaces are also called agglutinogens because they are partially responsible for Red Blood Cell agglutination in mismatched transfusions.A blood sample was taken from the voluntary students of Dilla University. There are differences in frequency distribution of the blood group (ABO) among the ethnic groups and administrate of the students at Dilla University. The most prevalent blood group was type O (44.49 %) followed by A (26.32 %), B (26.32 %), and AB (2.87 %). The most prevalent Rh-positive blood group among students were type O+ (41.94 %) followed by A+ (31.72 %), B+ (26.34 %), and AB+ (2.15 %) as well as the highest Rh-negative blood groups among students were type O- (65.22 %) followed by B- (26.08%), AB- (26.34 %), and no A- blood group. Therefore, the blood type and group of Dilla University students was varies across the ethnic groups.
Abstract: Red Blood cells (Erythrocytes) have an inherited combination of proteins, glycol-proteins, and glycol-lipids on their surfaces. These function as antigens that enable our immune system to distinguish our own cells from foreign invaders. Part of the immune response is the production of globulins called antibodies to combat the invader. In blood typi...
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