Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt
Hanaa Mahdy Abouzied,
Walid Mohamed El-Orabey,
Mohamed Abd El-Halim Abou-Zeid
Issue:
Volume 6, Issue 4, December 2018
Pages:
37-43
Received:
12 November 2018
Accepted:
4 December 2018
Published:
23 January 2019
DOI:
10.11648/j.ijgg.20180604.11
Downloads:
Views:
Abstract: Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.
Abstract: Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,6...
Show More
Identification of Novel Submergence Tolerant Local Rice Cultivars of Bangladesh
Al Amin,
Khandakar Md Iftekharuddaula,
Animesh Sarker,
Ashraf Hossain Talukder,
Sharmishtha Ghoshal,
A. K. M. Shalahuddin,
Tamal Lata Aditya,
Md Ansar Ali,
Bertrand Collard
Issue:
Volume 6, Issue 4, December 2018
Pages:
44-51
Received:
16 December 2018
Accepted:
28 December 2018
Published:
28 January 2019
DOI:
10.11648/j.ijgg.20180604.12
Downloads:
Views:
Abstract: Bangladesh had harbored numerous submergence tolerant rice local cultivars or landraces (popularly known as Joli Aman). As the local cultivars endure intervallic flash-flooding year after year, it is presumed that genes/Quantitative Trait Loci (QTLs) that give resilience are probably going to be available in these genotypes. In submergence screening, the performance of three tested accessions (Lakhi, Atshotti and Damsi) demonstrated them to be as tolerant as the check FR13A. Other germplasm accessions (DSL-78-8, Laldepa, Putidepa, Laxmi digha) performed great recuperation capacity after de-submergence. Damsi, Rajasail, Haloi, Lal Digha, Manik Digha, Bhawalia, DSL-78-8, Putidepa, Atshotti and Jalda IRRI don't have the SUB1 QTL and furthermore not like the BR5, a submergence susceptible check variety. Bajal, Horkoach, Pathornuti, Laxmi Digha, Horinga Digha, Bhawalia Digha and BR5 was not amplified by Gns2 conferring SUB1 QTL (submergence tolerance specific QTL). Significantly, the local cultivars i.e. DSL-78-8, Putidepa and Sadadanga boro were identified as having better submergence tolerance however having no resistant alleles of GnS2 and Sub1C173, indel markers specific to SUB1 QTL. The result of molecular screening revealed new submergence tolerant rice cultivars which do not possess SUB1 QTL. To discover new submergence tolerant QTLs other than SUB1 through QTL mapping, newly identified submergence tolerant germplasm can be utilized.
Abstract: Bangladesh had harbored numerous submergence tolerant rice local cultivars or landraces (popularly known as Joli Aman). As the local cultivars endure intervallic flash-flooding year after year, it is presumed that genes/Quantitative Trait Loci (QTLs) that give resilience are probably going to be available in these genotypes. In submergence screenin...
Show More
