Impact of Anthropogenic Activities on Genetic Diversity of Celtis zenkeri Engl in South-West Nigeria
Fasalejo Olamidayo,
Lawal Amadu,
Adekunle Victor
Issue:
Volume 9, Issue 2, June 2021
Pages:
31-35
Received:
4 December 2020
Accepted:
22 December 2020
Published:
23 April 2021
Abstract: Celtis zenkeri is a valuable tropical tree species for industrial and domestic purposes. For several years, exploitation of this species has not been sustainable and no reforestation or cultivation effort has been put in place both by governmental and non-governmental agencies. Anthropogenic activities have resulted in deforestation and subsequent reduction in the population size of important timber species. The research aim was to determine the impact of anthropogenic activities on genetic diversity of Celtis zenkeri in South-West Nigeria. Extensive sampling of Celtis zenkeri was carried out in four natural forest ecosystems. Two pristine forests (SNR, Akure forest and Osun Osogbo sacred grove) and two degraded forests (OA3 and Eda forest reserve). Young leaves were collected from each tree found in each site and preserved with silica gel for molecular analysis. In all, 130 accessions were sampled and were analyzed with five chloroplast microsatellite markers. In the result, allelic richness, which is a measure of genetic diversity and an indicator of a population's long-term potential for adaptability and persistence, was significantly higher in the two pristine forests than the two degraded forests. Statistically, a significant difference was not recorded for the observed heterozygosities (Ho) in the population of Celtis zenkeri in the pristine forests (Queen’s plot and Osun-Osogbo Sacred Grove) and a degraded forest (OA3). The formal indicates the possibility of conserving genetic diversity using traditional conservation approaches such as taboos and restrictions. Though OA3 is a degraded forest, the impact of the anthropogenic activities may not have been severe when compared with Eda forest reserve. At present, the scale of human activities in Eda forest reserve and OA3 could pose a serious threat to the future viability of the species if unchecked. Therefore, ex-situ conservation and sustainable harvesting of Celtis zenkeri are recommended.
Abstract: Celtis zenkeri is a valuable tropical tree species for industrial and domestic purposes. For several years, exploitation of this species has not been sustainable and no reforestation or cultivation effort has been put in place both by governmental and non-governmental agencies. Anthropogenic activities have resulted in deforestation and subsequent ...
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Frequency of Deletion of AZF Region of the Y Chromosome in Chhattishgarh, India
Manisha Barnwal Sinha,
Rima Dada,
Suprava Patel,
Apoorva Joshi,
Nilaj Bagde,
Human Prasad Sinha
Issue:
Volume 9, Issue 2, June 2021
Pages:
36-41
Received:
11 May 2021
Accepted:
2 June 2021
Published:
21 June 2021
Abstract: Background: In the work up of male infertility, Y chromosome microdeletion screening is crucial. PCR is a very sensitive technique to screen Y chromosome microdeletion. In the current study, Y chromosome microdeletion was detected by PCR based technique. To the best of our knowledge, no such study has been reported from Chhattishgarh state of India so far. Material and methods: A total of seventy-three subjects were enrolled for the study during the period of one year. Out of which forty-seven subjects were cases (infertile men with oligozoospermia and azoospermia) and twenty-five were controls (with normozoospermia and having child). Semen analysis was done in each case to evaluate spermatogenesis status. Sperm DNA fragmentation by sperm chromatin dispersion of cases with oligozoopermia was also performed to detect DNA fragmentation Index. Results: Y chromosome microdeletion was observed in one out of forty-seven infertile males who were oligozoospermic or azoospermic. The type of deletion was AZFbc. Thus 2.12% men among oligozoospermic or azoospermic men have Y chromosome microdeletion in Chhattisgarh. Conclusion: In Indian population, AZFbc deletion has been found to be the second commonest type of deletion. In our study, we have also found this as the only deletion. This test also provides etiological interpretation of male infertility to the patient. We believe that awareness about transmission of deleted gene to the offspring could prevent infertility up to certain extent in the affected couples.
Abstract: Background: In the work up of male infertility, Y chromosome microdeletion screening is crucial. PCR is a very sensitive technique to screen Y chromosome microdeletion. In the current study, Y chromosome microdeletion was detected by PCR based technique. To the best of our knowledge, no such study has been reported from Chhattishgarh state of India...
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