International Journal of Genetics and Genomics

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Vaccine Design for Marburg Virus Using VP35 Protein

Received: 22 October 2022    Accepted: 8 December 2022    Published: 28 December 2022
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Abstract

Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.

DOI 10.11648/j.ijgg.20221004.11
Published in International Journal of Genetics and Genomics (Volume 10, Issue 4, December 2022)
Page(s) 85-93
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

NTCP, ABCpred, NetMHC, Ellipro

References
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  • APA Style

    Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. (2022). Vaccine Design for Marburg Virus Using VP35 Protein. International Journal of Genetics and Genomics, 10(4), 85-93. https://doi.org/10.11648/j.ijgg.20221004.11

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    ACS Style

    Mohammed Yousif Mohammed; Mona Abdelrhman Mohamed Khaier; Nuha Agabna; Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int. J. Genet. Genomics 2022, 10(4), 85-93. doi: 10.11648/j.ijgg.20221004.11

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    AMA Style

    Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int J Genet Genomics. 2022;10(4):85-93. doi: 10.11648/j.ijgg.20221004.11

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  • @article{10.11648/j.ijgg.20221004.11,
      author = {Mohammed Yousif Mohammed and Mona Abdelrhman Mohamed Khaier and Nuha Agabna and Sania Shaddad},
      title = {Vaccine Design for Marburg Virus Using VP35 Protein},
      journal = {International Journal of Genetics and Genomics},
      volume = {10},
      number = {4},
      pages = {85-93},
      doi = {10.11648/j.ijgg.20221004.11},
      url = {https://doi.org/10.11648/j.ijgg.20221004.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20221004.11},
      abstract = {Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Vaccine Design for Marburg Virus Using VP35 Protein
    AU  - Mohammed Yousif Mohammed
    AU  - Mona Abdelrhman Mohamed Khaier
    AU  - Nuha Agabna
    AU  - Sania Shaddad
    Y1  - 2022/12/28
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijgg.20221004.11
    DO  - 10.11648/j.ijgg.20221004.11
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 85
    EP  - 93
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20221004.11
    AB  - Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.
    VL  - 10
    IS  - 4
    ER  - 

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Author Information
  • Department of Biotechnology, Faculty of Industrial and Applied Science, University of Bahri, Khartoum, Sudan

  • Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan

  • Department of Pharmacology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

  • Department of Pharmacology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

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