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Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure

Received: 31 August 2019     Accepted: 18 October 2019     Published: 26 October 2019
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Abstract

Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.

Published in International Journal of Genetics and Genomics (Volume 7, Issue 4)
DOI 10.11648/j.ijgg.20190704.14
Page(s) 110-114
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Colon Adenocarcinoma, Nanoparticles, Oxidative Stress, SOD, GS, CAT

References
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Cite This Article
  • APA Style

    Metin Budak. (2019). Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. International Journal of Genetics and Genomics, 7(4), 110-114. https://doi.org/10.11648/j.ijgg.20190704.14

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    ACS Style

    Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int. J. Genet. Genomics 2019, 7(4), 110-114. doi: 10.11648/j.ijgg.20190704.14

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    AMA Style

    Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int J Genet Genomics. 2019;7(4):110-114. doi: 10.11648/j.ijgg.20190704.14

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  • @article{10.11648/j.ijgg.20190704.14,
      author = {Metin Budak},
      title = {Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure},
      journal = {International Journal of Genetics and Genomics},
      volume = {7},
      number = {4},
      pages = {110-114},
      doi = {10.11648/j.ijgg.20190704.14},
      url = {https://doi.org/10.11648/j.ijgg.20190704.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190704.14},
      abstract = {Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure
    AU  - Metin Budak
    Y1  - 2019/10/26
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijgg.20190704.14
    DO  - 10.11648/j.ijgg.20190704.14
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 110
    EP  - 114
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20190704.14
    AB  - Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.
    VL  - 7
    IS  - 4
    ER  - 

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Author Information
  • Department of Biophysics, Trakya University, Edirne, Turkey

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