Volume 8, Issue 1, March 2020, Page: 1-10
Inheritance and Detection of Leaf Rust Resistance Genes in Some Egyptian Wheat Cultivars
Walid Mohamed El-Orabey, Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Ola Ibrahim Mabrouk, Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Mohamed AbdAllah Gad, Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Samar Mohamed Esmail, Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Received: Nov. 20, 2019;       Accepted: Dec. 11, 2019;       Published: Jan. 4, 2020
DOI: 10.11648/j.ijgg.20200801.11      View  82      Downloads  63
Resistance to leaf rust caused by Puccinia triticina can be improved in wheat cultivars by detection of leaf rust resistance genes that are present in the wheat cultivars. This study was carried out during 2016/17, 2017/18 and 2018/19 growing seasons to study the inheritance and genetic nature of wheat leaf rust resistance in eight Egyptian bread wheat cultivars i.e. Gemmeiza 9, Gemmeiza 10, Gemmeiza 11, Gemmeiza 12, Sids 1, Sids 12, Sids 13 and Sids 14. Crosses were done between the eight tested wheat cultivars and the highly susceptible wheat variety; Thatcher to determine the nature of leaf rust resistance and number of leaf rust resistance genes in these cultivars. Also, crosses were done between the tested wheat cultivars and six leaf rust monogenic lines i.e. Lr 50, Lr 51, Lr 54, Lr 64, Lr 67 and Lr 68 to determine the presence of these genes in the tested cultivars. The F1 seeds were planted to produce F2 plants. The resulted F2 plants were tested at adult plant stage at Behira governorate during 2018/19 growing season. Segregations of F2 plants at adult plant stages indicated that the wheat cultivar Gemmeiza 12 has the two leaf rust resistance genes; Lr 50 and Lr 67 and the wheat cultivar Sids 14 has one leaf rust resistance gene; Lr 64. While, the other tested wheat cultivars doesn’t carry any of the tested leaf rust resistance genes at adult-plant stage. We recommend using these leaf rust resistance genes in the breeding program to improve wheat resistance to leaf rust.
Wheat, Leaf Rust, Puccinia triticina, Resistance Genes
To cite this article
Walid Mohamed El-Orabey, Ola Ibrahim Mabrouk, Mohamed AbdAllah Gad, Samar Mohamed Esmail, Inheritance and Detection of Leaf Rust Resistance Genes in Some Egyptian Wheat Cultivars, International Journal of Genetics and Genomics. Vol. 8, No. 1, 2020, pp. 1-10. doi: 10.11648/j.ijgg.20200801.11
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Huerta-Espino J, Singh, RP, German S, McCallum BD, Park RF, Chen WQ, Bhardwaj SC and Goyeau H (2011). Global status of wheat leaf rust caused by Puccinia triticina. Euphytica, 179: 143-160.
Negm SS, Boulot OA and Herms GA (2013). Virulence dynamics and diversity in wheat leaf rust (Puccinia tritiina) populations in Egypt during 2009/2010 and 2010/2012 growing season. Egypt. J. of Appl. Sci., 28 (6): 183-212.
El-Orabey WM, Sallam ME, Omara R and Abd El-Malik NI (2015). Geographical distribution of Puccinia triticina physiologic races in Egypt during 2012-2014 growing seasons. African J. of Agric. Res., 10 (45): 4193-4203.
El-Orabey WM, Omara RI and Abou-Zeid MA (2018). Diversity and virulence dynamics within Puccinia triticina populations in Egypt. J. Plant Prot. Path., 9: 735-745.
Najeeb KMA, Thabet M, Negm SS and EL-Deeb SH (2019). Monitoring of puccinia triticina Erikss. Physiologic races and effectiveness of Lr-genes in Egyptian wheat during 2014-2016 growing season. International J. of Agric. Te. 15 (1): 35-54.
German SE, Barcellos A, Chaves M, Kohli M, Campos P and Viedma L (2007). The situation of common wheat rusts in the Southern Cone of America and perspectives for control. Aust. J. Agric. Res., 58: 620-630.
Negm SS (2004). Partial Resistance to Leaf Rust in Some Egyptian Wheat Varieties. Ph. D. Thesis, Fac. Agric., Minufiya Univ., 180 p.
Mabrouk OI (2016). Durable resistance to leaf rust in some Egyptian wheat cultivars. Ph. D. Thesis, Plant Pathology, Cairo Uni., 150 p.
Shahin SI and El-Orabey WM (2016). Assessment of grain yield losses caused by Puccinia triticina in some Egyptian wheat genotypes. Minufiya J. Agric. Res., 41: 29-37.
El-Orabey WM, Abd El-Malik NI, Ashmawy MA. and Abou-Zeid MA (2017). Reduction in grain yield caused by leaf rust infection in seven Egyptian wheat cultivars. Minufiya J. Plant Protect., 2: 71-81.
Thabet M and Najeeb KMA (2019). Impact of wheat leaf rust severity on grain yield losses in relation to host resistance for some Egyptian wheat cultivars. Middle East j. of Agr. Res., 6 (4): 1501-1509.
El-Orabey WM, Hamweih A and Ahmed SM (2019). Molecular markers and phenotypic characterization of adult plant resistance genes Lr 34, Lr 46, Lr 67 and Lr 68 and their association with partial resistance to leaf rust in wheat. J. of Genetics, 98: 1-12.
Singh D, Park RF and McIntosh RA (2008). Characterisation of wheat leaf rust resistance gene Lr 34 in Australian wheat using components of resistance and the linked molecular marker csLV 34. Australian J. Agric. Res., 58 (11): 1106-1114.
Line RF and Chen XM (1989). Expression of stripe rust resistance genes in differential wheat cultivars. Phytopathol., 82 (12): 1428 1434.
Sallam ME, El-Orabey WM, and Omara RI (2016). Seedling and adult plant resistance to leaf rust in some Egyptian wheat genotypes. African J. of Agric. Res., 11 (4): 247-258.
Bansal UK, Bossolini E, Miah H, Keller B, Park RF, Bariana HS (2008). Genetic mapping of seedling and adult plant stem rust resistance in two European winter wheat cultivars. Euphytica, 164: pp 821-828.
Roelfs AP, Singh RP and Saari EE (1992). Rust diseases of wheat. Concepts and methods of disease management. CIMMYT, Mexico, D. F.
Tervet I and Cassell RC (1951). The use of cyclone separation in race identification of cereal rusts. Phytopathology, 4: 282-285.
Peterson RF, Campbell AB and Hannah AE (1948). A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can. J. Res., 60: 496-500.
Singh RP and Huerta-Espino J. (1995). Inheritance of seedling and adult plant resistance to leaf rust in wheat cultivars Ciano 79 and Papago 86. Plant Dis., 79: 35-38.
Steel RGD. and Torrie JH (1960). Principles and Procedures of Statistics. McGraw-Hill Book Company, New York. 481 pp.
Wright S (1968). Evolution and Genetics of Populations. Vol. I. Genetic and Biometric Foundations. University of Chicago Press, Chicago, 480p.
Romero GE and Frey KJ (1973). Inheritance of semi dwarfiness in several wheat crosses. Crop Sci., 13: 334-337.
Lush JL (1949). Heritability of quantitative characters in farm animals. Hereditas, Supp. Vol. pp. 356-375. (cited after Weber Agro. J., 44: 202-209).
Park R (2008). Breeding cereals for rust resistance in Australia. Plant Pathology, 57 (4): 591-602.
Bjarko ME and Line RF (1988). Inheritance of latent period, infection type and area under the disease progress curve in two slow leaf rusting spring wheat cultivars. Phytopathol., 78: 457-461.
Boulot OA (1997). Further Studies on Leaf Rust of Wheat. Ph. D. Thesis, Fac. Agric. Minufiya University, 290 p.
Da-Silva PR, Brammer SP, Guerra DS, Milach CK, Barcellos AL and Baggio MI (2012). Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi. Genet. Mol. Res., 11 (3): 2823-2834.
Loladze A, Kthiri D, Pozniak C and Ammar K (2014). Genetic analysis of leaf rust resistance in six durum wheat genotypes. Phytopathol., 104 (12): 1322-1328.
Kolmer JA (1996). Genetics of resistance to wheat leaf rust. Annu. Rev. Phytopathol., 34: 435-455.
Brown-Guedira G, Singh S, and Fritz A (2003). Performance and mapping of leaf rust resistance transferred to wheat from Triticum timopheevii sub sp. armeniacum. Phytopathology, 93: 784-789.
Pestsova E, Ganal MW, Röder MS (2000). Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 43 (4): 689-697.
Dvorak J, and Knott DR (1980). Chromosome location of two leaf rust resistance genes transferred from Triticum speltoides to T. aestivum. Can. J. Genet. Cytol., 22: 381–389.
Dvorak J. (1977). Transfer of leaf rust resistance from Aegilops speltoides to Triticum aestivum. Canadian Journal of Genetics and Cytology, 19: 133-141.
Moore JW, Herrera-Foessel S, Lan C, Schnippenkoetter W, Ayliffe M, Huerta-Espino J et al. (2015). A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat. Genet., 47: 1494-1498.
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L et al. (2012). Lr 68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor. Appl. Genet. 124, 1475–1486.
Pinto da Silva GBP, Zanella CM, Martinelli JA, Chaves MS, Hiebert CW, McCallum BD et al. (2018). Quantitative trait loci conferring leaf rust resistance in hexaploid wheat. Phytopathology, 108: 1344-1354.
Fahmi AI, Nazim M, Khalifa SZ and El-Orabey WM (2003). Identification of seedling leaf rust resistance genes in Egyptian wheat cultivars. Egyptian J. of Genetics and Cytology, 32: 357-373.
Fahmi AI, Nazim M, Khalifa SZ and El-Orabey WM (2005). Genetics of adult plant resistance to leaf rust in Egyptian wheat. Egyptian J. of Phytopath., 33: 1-10.
El-Orabey WM and Nagaty HH (2013). Detection of the leaf rust resistance gene Lr 9 in some Egyptian wheat varieties. Minufiya J. Agric. Res., 38: 895-907.
Hasan MA, El-Orabey WM, El-Naggar DR and Ashmawy MA (2014). Genetic analysis of seedling and adult plant resistance to leaf rust in nine bread wheat cultivars. Zagazig J. of Agric. Res., 41 (6): 1: 12.
Shahin SI and El-Orabey WM (2015). Relationship between partial resistance and inheritance of adult plant resistance gene Lr 46 of leaf rust in six bread wheat varieties. International J. Science and Research, 4 (1): 1511-1517.
Fahmi AI, El-Shehawi AM and El-Orabey WM (2015). Leaf rust resistance and molecular identification of Lr 34 gene in Egyptian wheat. Journal of Microbial & Biochemical Technology, 7 (6): 338-343.
Lukasz S, Golka L and Chelkowski J (2003). Leaf rust resistance genes of wheat: identification in cultivars and resistance sources. J. Appl. Genet., 44: 139-149.
Boskovic J, Boskovic M and Prijic Z (2008). Pyramiding major genes for resistance to leaf rust pathogen of wheat. Proc. of the International Scientific Conference on Multifunctional Agriculture. University of Szeged, Faculty of Agriculture, Hungary. p. 24.
Elbasyoni IS, El-Orabey WM, Morsy S, Baenziger PS, Al Ajlouni Z and Dowikat I (2019). Evaluation of a global spring wheat panel for stripe rust: Resistance loci validation and novel resources identification. PLOS ONE 14 (11): 1-20.
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