Volume 5, Issue 6, December 2017, Page: 76-84
High-Throughput Sequencing Reveals miRNAs Affecting Follicle Development in Chicken
Quan Zhang, Department of Animal Breeding and Genetics, Agricultural College, Guangdong Ocean University, Zhanjiang, P. R. China
Shanshan Li, Department of Animal Breeding and Genetics, Agricultural College, Guangdong Ocean University, Zhanjiang, P. R. China
Bingwang Du, Department of Animal Breeding and Genetics, Agricultural College, Guangdong Ocean University, Zhanjiang, P. R. China
Received: Oct. 5, 2017;       Accepted: Oct. 19, 2017;       Published: Nov. 16, 2017
DOI: 10.11648/j.ijgg.20170506.12      View  2153      Downloads  102
Abstract
As the derivative of chicken skin, hair follicle is capable of self-renew. Its proliferation and differentiation result in hair formation. MicroRNAs (miRNAs) can effectively regulate gene expression at the post-transcriptional level and play a critical role in tissue growth, development. In this study, we used next generation sequencing technology sequenced miRNAs of the hair follicle derived from the 13 day-old chicken (Gallus gallus) embryos in which from Kirin chicken and Huaixiang chicken that feathers having morphogenesis with significantly different curling. A population of conserved miRNAs was identified. These conserved miRNAs were derived from 638 homologous hairpin precursors across 5 animal species. We identified a total of 645 miRNAs in the chicken embryos. Among them, 11 differentially expressed miRNAs were identified (> ±2 Fold, p value <0.05) by comparing Kirin chicken and Huaixiang chicken. Several gene ontology (GO) biology processes and the WNT, BMP and TGF-β signaling pathways were found to be differentially expressed miRNAs as part of hair follicle development process. The miR-1623 has an effect on WNT4 and involved in hair follicle cell development. This study has identified miRNAs that associated with the chick embryonic hair follicle development and identified some target miRNAs for further research into their role played in feather growth.
Keywords
Chicken Embryo, Follicle, Mirnas
To cite this article
Quan Zhang, Shanshan Li, Bingwang Du, High-Throughput Sequencing Reveals miRNAs Affecting Follicle Development in Chicken, International Journal of Genetics and Genomics. Vol. 5, No. 6, 2017, pp. 76-84. doi: 10.11648/j.ijgg.20170506.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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